Analysis Note

ControlMCF-7 cell lysate

Application

Research CategorySignaling

This Anti-ODC1 Antibody, clone CPTC-ODC1-2 is validated for use in WB for the detection of ODC1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ornithine decarboxylase (ODC1) is the first enzyme in polyamine synthesis and is mandatory for normal growth and is increased in many types of cancers. ODC1 has been shown to suppress the fmc1 deletion in yeast and restores healthy respiratory growth. ODC1 may play a role in promoting DNA synthesis in hepatocytes.

Immunogen

Full length of recombinant protein corresponding to human ODC1.

Material obtained from the National Cancer Institute's Clinical Proteomic Technologies for Cancer (CPTC) program.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 10 µg/mL of this antibody detected ODC1 on 10 µg of MCF-7 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~51 kDa observed. A band at ~100 kDa may be observed in some lysates and may be the homodimer of this protein.